Chemical used for preparation of clinical reagent are of standard quality (Merck and Qualigens.) Weighing scale used to prepare this reagent is of high standard accuracy with least count 0.1 mg (Metteler Toledo AB-204). The water which is most important solvent used to prepare clinical reagent. Are of type-1 electronic grade water produce through ELGA system from UK to prepare reagent Glassware used for preparation are of class A.